RunView Safe Series for - Midi-Mini electrophoresis systems

  • Portable adjustable blue light illuminator for use with MSMINI and MSMIDI electrophoresis
    tanks.
  • Blue light is completely safe to both operator and DNA alike, and results in improved cloning
    efficiency compared to UV.
  • Emission filter lid with built in extractor fan enables condensation-free viewing of blue-light fluorescent gels, containing SYBR Green, SYBR
    Safe and runSAFE stains
  • runDOC-MINI enables visualisation of real-time electrophoresis of MSMINI and MSMIDI gels using adjustable blue light illuminator
  • Electrophoresis kit available
    containing agarose, 10x Trisborate
    electrophoresis buffer, 1kb DNA markers and runSAFE

Continuing from the success of the award-winning runVIEW™ system, the runVIEW™ MINI and MIDI are Cleaver Scientific’s latest additions to its Safe Range of electrophoresis products. Both systems benefit from the blue light illumination of fluorescently stained 7- or 10-cm-wide agarose gels to allow users to view the size-fractionation of nucleic acids in real-time. An adjustable blue-light illuminator platform accommodates both the MSMINI and MSMIDI electrophoresis tanks, while band
visualisation is achieved through the corresponding lid containing an amber spectral emission filter. Each lid remains free of condensation by a built-in extractor fan and may be connected either to the user’s own power supply or to the optional, but recommended, nanoPAC-300.

Real-time size fractionation and recovery of nucleic acids
runVIEW is an innovative new system which can be used to maximise the efficiency of DNA recovery from EtBr and SYBR stained gels by minimising the number of steps involved in postelectrophoretic purification. runVIEW consists of a multiSUB horizontal electrophoresis unit with a bluVIEW lid, containing an orange spectral emission filter within its viewing pane, and a base unit with a blue LED gel illuminator.

Simple DNA recovery
Using runVIEW is simple: first, cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied, before transferring the blue-light transparent gel tray to the tank located on the base unit. Add sufficient buffer just to cover the gel, and remove the combs to load the DNA samples into the upper row of wells (‘Loading’ tier). Replace the lid to connect the tank to the power supply before applying the voltage and switching on the blue LED gel illuminator. Watch through the bluVIEW lid’s viewing pane as the samples migrate in real-time to the second row of wells (‘extraction’ tier). Once the DNA bands of interest enter the ‘extraction’ tier, simply stop the power supply, remove the lid and harvest the DNA by pipette.

Track DNA without harmful UV
Blue light is safe and has none of the detrimental effects of UV transillumination, including mutagenesis which can compromise cloning effi ciency, while runVIEW’s capacity to provide real-time visualisation of electrophoresis enables DNA to be tracked as it migrates through the gel. This allows the user to judge precisely when the band of interest is ready for extraction.

Save on time-consuming gel elution techniques
Running DNA into a well containing buffer effectively purifi es the DNA of agarose, eliminating the need for time-consuming gel excision and purifi cation techniques that also result in sample loss. Once harvested, the DNA needs only to be cleaned by a straightforward ethanol precipitation when it is ready for further digestion or ligation.

No expensive commercial gels
runVIEW works with standard EtBr, SYBR Green and SYBR Safe gels cast within the MS Midi or Mini gel trays, and therefore does not require expensive precast gels and accessories.


RunView Mini Midi Electrophoresis Specifications

Common
Blue Light
Wavelength
470nm Timer 1-999 minutes with alarm
Voltage/
Resolution
25-150V / 1V Safety Device No load detection
Current/
Resolution
400mA / 1mA Operating
Temperature
>Ambient to 40°C
Power 30 W    
Operating Mode Constant Voltage
or Current
Rated Voltage 100-240V, 50/60Hz
RunVIEW Gel System MINI
Gel Dimensions
(W X L)
7 x 7, 7 x 10 cm
bluVIEW
Lid Design
Amber spectral emission filter with built-in extractor fan powered by the base unit
Unit dimensions
(W X D X H)
9 x 21 x 9cm    
Buffer volume 225ml Combs 1mm x 8 samples (two included)
RunVIEW Gel System MIDI
Gel Dimensions
(W X L)
10 x 7, 10 x 10 cm
bluVIEW
Lid Design
Amber spectral emission filter with built-in extractor fan powered by the base unit
Unit dimensions
(W X D X H)
12.5 x 22 x 9cm    
Buffer volume 300ml Combs 1mm x 16 sample (two included)

Ordering information for RunView Midi Mini

  Complete systems with power supply and chemicals  
CSL-RVMSMINI-CK CSL-RVMSMINI-C plus reagent kit for real-time
electrophoresis (includes: CSL-RUNSAFE [1ml runSAFE stain,], CSL-MDNA [1kb DNA ladder, ], CSL-AG100 [100g agarose, ], & CSL-TBEP [Powdered Tris-Borate-EDTA Running Buffer – 10 pouches,
$1650.00
CSL-RVMSMIDI-CK CSL-RVMSMIDI-C plus reagent kit for real-time electrophoresis (includes: CSL-RUNSAFE [1ml runSAFE stain,], CSL-MDNA [1kb DNA
ladder,], CSL-AG100 [100g agarose, ], & CSL-TBEP [Powdered Tris-Borate-EDTA Running Buffer – 10 pouches])
$1695.00
  If you already have a power supply  
CSL-RVMSMINI-S CSL-RVBSBVLID-MINI plus MSMINIDUO tank with 7x7 & 7x10cm trays, 1 set of casting dams and 2x 8-sample combs (No Power supply) $1150.00
CSL-RVMSMIDI-S CSL-RVBSBVLID-MIDI plus MSMIDIDUO tank with 10x7 & 10x10cm trays, 1 set of casting dams and 2x 16-sample combs. (no power supply) $1195.00