Cleaver Scientific’s ‘Safe Series’ has been developed to
address the needs of researchers looking to work with safer
alternatives to UV irradiation and ethidium bromide, both of which are known to have harmful mutagenic effects. The award-winning* runVIEW™ electrophoresis system, now part of the ‘Safe Series’ includes the runDOC imaging system and its repertoire of runSAFE non-mutagenic, blue light fluorescent stains.
Real-time size fractionation and recovery of
runVIEW is an innovative new system which can be used to maximise the efficiency of DNA recovery from EtBr and SYBR stained gels by minimising the number of steps involved in postelectrophoretic purification. runVIEW consists of a multiSUB MSCHOICE horizontal electrophoresis unit with a bluVIEW lid, containing an orange spectral emission filter within its viewing pane, and a base unit with a blue LED gel illuminator. The power supply for the system is integrated into the base unit.
Simple DNA recovery
Using runVIEW is simple: first, cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied, before transferring the blue-light transparent gel tray to the MSCHOICE tank located on the base unit. Add sufficient buffer just to cover the gel, and remove the combs to load the DNA samples into the upper row of wells (‘Loading’ tier). Replace the lid to connect the MSCHOICE tank to the integrated power supply before applying the voltage and switching on the blue LED gel illuminator. Watch through the bluVIEW lid’s viewing pane as the samples migrate in real-time to the second row of wells (‘extraction’ tier). Once the DNA bands of interest enter the ‘extraction’ tier, simply stop the power supply, remove the lid and harvest the DNA by pipette.
Track DNA without harmful UV
Blue light is safe and has none of the detrimental effects of UV transillumination, including mutagenesis which can compromise cloning effi ciency, while runVIEW’s capacity to provide real-time visualisation of electrophoresis enables DNA to be tracked as it migrates through the gel. This allows the user to judge precisely when the band of interest is ready for extraction.
Save on time-consuming gel elution techniques
Running DNA into a well containing buffer effectively purifi es the DNA of agarose, eliminating the need for time-consuming gel excision and purifi cation techniques that also result in sample loss. Once harvested, the DNA needs only to be cleaned by a straightforward ethanol precipitation when it is ready for further digestion or ligation.
No expensive commercial gels
runVIEW works with standard EtBr, SYBR Green and SYBR Safe gels cast within the 15x7, 15x10 or 15x15cm MSCHOICE gel trays, and therefore does not require expensive precast gels and accessories.
A self-contained system
The base unit, which houses the in-built power supply and blue LED gel illuminator, is compact, dual-voltage and portable, and allows electrophoresis, gel visualisation and extraction to be performed at the bench, without the inconvenience of having to transport gels to a darkroom elsewhere within the laboratory.
Other benefits offered by runVIEW include:
• Power supply integrated within the base unit – adjustable in precise 1V or 1mA increments to a maximum 150V or 300mA constant voltage or current output; timer function to 999 minutes for extended runs.
• Specialist combs for specialist applications - double-sided 1mm preparatory combs (1- / 2-sample and 4- / 16-sample standard) included for nucleic acid recovery, plus four multichannel compatible 20- / 28-sample combs for rapid screening of nucleic acids from 96-well thermal cycler blocks and microtitre plates.
• Get real-time - existing MSCHOICE users may customise their system by purchasing the base station and bluVIEW lid.
• Versatile - Both power supply and gel illuminator can be used with other gels and electrophoresis systems.
Gel Documentation option:
RunDOC is a portable, lightweight gel documentation system designed exclusively for use with RunVIEW – the innovative new system designed for real-time size fractionation and recovery of nucleic acids. RunDOC comprises a darkroom hood and 12.1 megapixel digital camera to capture images of nucleic acid gels stained with EtBr, SYBR and runSAFE. The RunDOC darkroom hood is placed directly over the RVMSCHOICE gel tank located on the RunVIEW base unit, which includes an integrated power supply and blue LED gel illuminator. The blue LED illuminator provides the excitation source for the DNA gel located within the tank, and following excitation the light emitted is then visualized using either one of the bluVIEW lid options (supplied with RunVIEW and used with the RVMSCHOICE gel tank) or the RunDOC filter slide. RunDOC also benefits from a small footprint area, occupying minimal space within the laboratory. -
|runVIEW Viewing Dock|
|470nm||Timer||1-999 minutes with alarm|
|25-150V / 1V||Safety Device||No load detection|
|300mA / 1mA||Operating
|>Ambient to 40°C|
|Power||30 W||Dimensions||293 x 220 x 80 mm|
|Operating Mode||Constant Voltage
|Rated Voltage||100-240V, 50/60Hz|
|RunVIEW Gel System|
(W X L)
|15 x 7, 15 x 10 &
15 x 15cm
|Orange spectral emission fi lter with
condensation-free viewing panel
(W X D X H)
|26.5 x 17.5 x 9cm||Included
|2x 1-sample / 2-sample preparatory;
2x 4-sample preparatory /
4x 20- /28- sample multichannel
|Buffer volume||500ml||Comb Thickness||1mm|
|CSL-RVMSCHOICE7||runVIEW system complete with
15 x 7cm gel tray, 1 set of casting
dams and 8 double-sided combs
|CSL-RVMSCHOICE10||runVIEW system complete with
15 x 10cm gel tray, 1 set of casting
dams and 8 double-sided combs
|CSL-RVMSCHOICE15||runVIEW system complete with
15 x 15cm gel tray, 1 set of casting
dams and 8 double-sided combs.
|CSL-RVMSCHOICETRIO||runVIEW system complete with
15 x 7, 15 x 10 and 15 x 15cm gel
trays, 3 sets of casting dams and 8
|CSL-RVBSBVLID||runVIEW base station & bluVIEW lid.||$1520.00|
|CSL-RVGELDOC||runVIEW gel documentation hood
with 10MP camera