SERVA PRECOTES™/PreNets™/FocusGels
Precast Polyacrylamide Gels for IEF


  • Ready to use, no gel pretreatment required
  • High resolution, sharp bands, reproducible results
  • Short focusing times
  • Fast staining and destaining properties
  • Various gel formats, thicknesses and pH fractions available
  • Gel can be cut to size
  • Safe and gentle handling of SERVALYT™ PreNets TM gels for subsequent blotting
  • Easy to dry (in air) for documentation purposes
  • Can be adapted to any pH-range desired by rapid equilibration
  • Suited for IEF in the basic pH-range
  • Suited for IEF in the presence of urea
  • Excellent resolution
  • Long shelf life
  • Blotting gels available

Applications

Blank PRECOTES™ were developed by SERVA to provide a versatile solution to perform isoelectric focusing (IEF) of any pH range. Blank PRECOTES™ are thin (0.3 mm) polyacrylamide gels cast onto GEL-FIX™ support film that contain only BisTris buffer pH 6.5. They are given the prefix »blank« to indicate that they are (almost) »empty« gels with a matrix that can be adapted to anything the user wants it to be.
Blank PRECOTES™ are equilibrated in the ampholyte mixture of choice prior to electrophoresis. Shelf-life of Blank PRECOTES™ is at least 12 months, either as blank gels (without ampholyte) or in the equilibrated form (with ampholyte, without urea).

SERVALYT™ PRECOTES™ gels are ready to use and designed for horizontal flat bed electrophoresis. The gels consist of thin polyacrylamide layers bound to an inert polyester support film (GEL-FIXTM) which provides good mechanical stability and easy handling.
In order to prevent the precast gels from drying up and becoming damaged, the surface is covered with a thin film (GEL-FIXTM for Covers). Each SERVALYT™ PRECOTES™ gel is packed separately. Refer to the package label for the shelf life of unopened product.
Recommended storage temperature is 2 to 8°C (35°F - 46°F).
SERVALYT™ PreNets™ are thin ready-to-use gels prepared from acrylamide poured onto an inert polyester film, similar to SERVALYT™ PRECOTES™ but with the gel layer not covalently bound to the film. The SERVALYT™ PreNets™ gel layer can be removed after electrophoresis by lifting it from the film. It can then be transferred to a blotting device to carry out electrotransfer onto a membrane. SERVALYT™ PreNets™ contain an additional support fabric (polyester net, NetFix™) cast into the gel layer which provides mechanical support and stabilizes the gel layer. This makes it very easy to handle the thin gel layer, without risking damage to the gel (tearing, etc.) when transferring it (and your valuable samples!) to a blotter.

Blank PRECOTES ™ were developed by SERVA to provide a versatile solution to perform isoelectric focusing (IEF) of any pH range. Blank PRECOTES™ are thin (0.3 mm) polyacrylamide gels onto GEL-FIXTM support film that contain only Bis-Tris buffer pH 6.5. They are given the prefix “blank” to indicate that they are (almost) “empty” gels with a matrix that can be adapted to anything the user wants to be. Blank PRECOTES™ get equilibrated in the ampholyte mixture of choice for only 30 minutes prior to electrophoresis. Resolution is at least as excellent as compared to the resolution obtained with gels of “cast-in” ampholyts.

When blotting is to be performed Blank PreNetsTM are the gels of choice. PreNetsTM gels are prepared with NetFixTM cast into the polyacrylamide matrix adding mechanical stability to the thin gel layer. NetFixTM is an inert polyester net activated to bind polyacrylamid.
After electrophoresis, the gel is lifted easily from its support film (non-binding) and transferred to the membrane of choice, no tearing or damage will occur when handling the thin layer due to the stabilizing effect of the built-in NetFixTM. Both methods, semidry and tank transfer, are applicable. Transfer using a Semidry blotter is usually completed within 30 minutes. Handling of PreNetsTM is identical in all steps to operating PRECOTESTM, including staining and drying.

Properties of SERVA Precast Gels for IEF SERVALYT™ PRECOTES™ SERVALYT™
PreNets™
Blank PRECOTES™ Blank PreNets™
Polymer concentration 5 % (T) 5 % (T) 5 % (T) 5 % (T)
Crosslinker (N,N´-methylene- bisacrylamide) 3 % (C) 3 % (C) 3 % (C) 3 % (C)
SERVALY™ concentration 5 % 5 % - -
Support film thickness (GEL-FIX™ for PAGE)
180 µm - 180 µm -
Cover film thickness (GEL-FIX™ for Covers) 75 µm 75 µm 75 µm 75 µm
Polyacrylamide gel layer

0.15 mm
0.3mm

0.3mm 0.3mm 0.3mm
Recommended storage temp 4 °C 4 °C 4 °C 4 °C
Shelf life 18 months 18 months 18 months 18 months
Gel Formats
Large size 245 x 125 mm   245 x 245 mm  
Standard size 125 x 125 mm 125 x 125 mm 125 x 125 mm 125 x 125 mm
Delivery/packaging        
Large size (245 x 125 mm) 5 gels/package 5 gels/package 5 gels/package 5 gels/package
Standard size (125 x 125 mm) 5 gels/package 5 gels/package 5 gels/package 5 gels/package

 

Ordering Information

SERVA offers a broad range of precast polyacrylamide gels for horizontal isoelectric focusing. The poylacrylamide layer is either bound to a sturdy support GEL-FIX™, an inert polyester film, activated to bind irreversibly to polyacrylamide, or to NetFix™, an inert polyester fabric, activated to bind polyacrylamide but open to both sides of the gel. NetFix™ is particularly useful when blotting is applied after electrophoresis as it stabilizes the gel but leaves both gel surfaces open, suitable for transfer. The surface of each precast gel is protected with a thin cover sheet (GEL-FIX™ Covers) against damage and drying out. SERVA precast gels are separately packed, each individually sealed in an aluthen bag which adds to longevity. SERVA precast gels are ready-to-use and offered with thin and ultrathin gel layers.

PAG Layer Size Qty Cat No  
SERVALYT™ PRECOTES™ Wide Range pH 3 - 10
150 μm 125 x 125 mm 5 pieces 42965.03 $284.00
150 μm 245 x 125 mm 5 pieces 42967.02 $393.00
300μm 125 x 125 mm 5 pieces 42866.02 $284.00
300 μm 245 x 125 mm 5 pieces 42867.02 $393.00
SERVALYT™ PRECOTES™ Wide Range pH 3 - 6
150 μm 125 x 125 mm 5 pieces 42974.02 $284.00
150 μm 245 x 125 mm 5 pieces 42919.03 $393.00
3000 μm 125 x 125 mm 5pieces 42874.02 $284.00
SERVALYT™ PRECOTES™ Wide Range pH 4 - 6
300 μm 125 x 125 mm 5 pieces 42875.02 $284.00
SERVALYT™ PRECOTES™ Wide Range pH 6 - 9
150 μm 125 x 125 mm 5 pieces 42878.02 $284.00

300 μm

125 x 125 mm 5 pieces 42978.02 $284.00

SERVALYT™ PreNets™ for subsequent blotting. They are precast gels, used in the same manner as the related SERVALYT™ PRECOTES ™ except that the gel, supported by a NetFix™ polyester fabric, is permeable for electrotransfer. The gel layer is not covalently bound to the backing and is lifted off easily.

PAG Layer Size Qty Cat No  
SERVALYT™ PRNets™ Wide Range pH 3 - 10
300 μm 125 x 125 mm 5 pieces 42738.02 $355.00
SERVALYT™ PRENets™ Wide Range pH 4 - 6
300 μm 125 x 125 mm 5 pieces 42748.02 $355.00

FocusGels
0.65 mm thick precast polyacrylamide gel, bound to GEL-FIX™ support film for IEF. The gels are non-toxic, because catalysts and other non-polymerized substances like acrylamid monomers are removed from the matrix. They contain a special SERVALYT™ cocktail designed to achieve an optimal pH gradient. Electrode solutions and electrode strips are not required, the electrodes
are placed directly on the gel surface. Samples are applied to the gel by pipetting the protein solution into preformed slots or by using applicator strips.

PAG Layer Qty Cat No Price
FocusGels 250 x 115 x 0.65mm      
pH 3-10 5 pieces 43327.01 $585.00
pH 3-10 24 slots 5 pieces 43335.01 $585.00
pH 6-11 24 slots 5 pieces 43329.01 $585.00
pH 6-11 40 slots 5 pieces 43333.01 $585.00
pH 3-7 5 pieces 43328.01 $585.00
pH 3-7 24 slots 5 pieces 43387.01 $585.00
pH 4-5 24 slots 5pieces 43332.01 $585.00
pH 4-6 24 slots 5 pieces 43334.01 $585.00
pH 6-9 Hemoglobin 24 slots 5 pieces 43330.01 $585.00

CSF Analysis Kit for PhastSystem™, 50 mm x 42 mm x 0.43 mm

1kit 43393.01 $459.00

Blank PRECOTES™ are thin (0.3 mm) polyacrylamide gels cast onto GEL-FIX™ support film that contain only BisTris buffer pH 6.5. They are given the prefix »blank« to indicate that they are (almost) »empty« gels with a matrix that can be adapted to anything the user wants it to be. Blank PRECOTES™/ PreNets™ are equilibrated in the ampholyte mixture of choice prior to electrophoresis.
Shelf-life of Blank PRECOTES™/ PreNets™ is at least 12 months, either as blank gels (without ampholyte) or in the equilibrated form (with ampholyte, without urea).

Blank Precotes™ PRNets™ Size Qty Cat No  
Blank PreCotes 300 μm 125 x 125 x 0.3 mm 5 pieces 42759.01 $166.00
Blank Precotes 300μm 245 x 125 x 0.3 mm 5 pieces 42710.01 $237.00
Blank PreNet 300 μm 125 x 125 x 0.3 mm 5 pieces 42758.01 $183.00