2D - Electrophoresis - tube gel systems
 1) Outer buffer chamber.
 2) Slab gel system. (2nd dimension)
 3) Capillary Module (1st dimension)
Isoelectric focusing is used for amphoteric  molecules  (having positive or negative charge depending on the pH) such as proteins, enzymes and amino acids. The Scie-Plas 2D Electrophoresis system comprises everything needed to run 2D electrophoresis. Both tube gels (first dimension) and vertical slabs (second dimension) can be run using these units.
    With the Modules available, 2 slab gels, or 10 capillary gels can be run in the tank at any one time. The modular and 2-D systems are available in two sizes 10 x 10 cm and 20 x 20 cm to complement the TV series Vertical gel electrophoresis systems.
First Dimension - Capillary Tube Gel Module
The Scie-Plas Capillary Gel Module which performs the first phase of 2-D electrophoresis (Isoelectric Focusing - IEF), allows 10 capillary gels to be run at any one time, using either the 10 x 10cm or 20 x 20cm TV systems. Essentially these gels differ in the length of the capillary tubes, which are 80 or 170 mm long, allowing a high degree of versatility in the separation desired. The capillary tubes have a wide capillary bore diameter, which greatly eases the extraction of the capillary gel once the first dimension - IEF phase is completed. After focusing, which typically takes between 3 to 4 hours, the tube gel can then be extracted and the samples separated on a slab gel for the second dimension, 2-D, size determining phase. The second separation can take as little as 1 hour to complete and therefore a complete 2-D separation can take as little as 4 hours.
Second Dimension - Vertical Slab Gel Module
The 10 x 10 cm and 20 x 20 cm vertical electrophoresis unit can run two gels or commercially available cassettes for the separation of nucleic acids or proteins. The running tank of this unit accepts the Capillary gel module and the tube gel module. The running buffer can be pre-chilled prior to electrophoresis or additional cooling can be achieved by using a bottom cooling block, The super-cooled versions are set up for use with a chiller circuite. Gels can be poured directly into the gel running frame which connects them to the top buffer tank. A leak proof seal is obtained with sprung clamping bars and screws. The complete gel assembly is then placed in the bottom buffer tank and can be fully submerged with the running buffer.
  TV100YK-2DSYS TV400YK-2DSYS
Unit Dimensions 28x15x18cm 38x15x27.5cm
Inner Tank Dimensions 16.5x11x15cm 27x12.5x25cm
Plate Dimensions 10x10x0.2cm 20.5x20x0.4 cm
Standard Spacer Dimensions 1x10x0.1cm 2x20x0.1cm
Active Gel Dimensions 8x8.5cm 16.5x17.5cm
Sample Capacity 2x1 / 2x12 2x1/ 2x24
Recommended Buffer Volume (PAGE GRM) Inner buffer chamber: 90ml Inner buffer chamber: 700ml
Gel Tank: 1600ml Gel Tank: 4200ml
Recommended Running Conditions for SDS-PAGE Voltage: 100-150V (10-15V/cm) 200-300V (10-15V/cm)
Current: 10-15mA 30-40mA
Time: 1-1.75h 2-2.5h
Capillary Dimensions Inner Diameter:1mm 1mm
Outer diameter: 5mm 5mm
Length: 80mm 170mm
Recommended Buffer Volume (CAPGRM) Upper Buffer Chamber:200ml 200ml
Gel tank: 1600ml 1600ml
Recommended Running conditions for IEF of Capillary Gels
  Pre-run Separation End of run
Voltage 200V 400V 500V
Time 0.5h 3h 0.5h
Volt-hours 100Vh 1200Vh 250Vh
  Pre-run Separation End of run
Voltage 200V 400V 500V
Time 0.5h 6 to 18h 0.5h
Volt-hours 100Vh 2400Vh - 7200Vh 250Vh
Snap-lock connectors for Cooling Coil Inner diameter : 10mm
Outer Diameter: 12mm
Inner diameter : 10mm
Outer Diameter: 12mm
Quick-fit Tubing Inner diameter : 10mm
Outer Diameter: 12mm
Inner diameter : 10mm
Outer Diameter: 12mm
Casting Base Silicone Seal Dimensions 1.5x11x0.8cm 1.7x22.5x0.8cm
Power Ouput Connectors shrouded, 4mm shrouded, 4mm
Recommended power supplies Consort EV215 or EV261 Consort EV215 or EV261

 

Ordering Information 2D Electrophoresis Systems
Mini 2D Electrophoresis System 
TV100YK-2DSYS

2D Electrophoresis system includes
TV100YK 10 cm Compact Dual Cooled Unit
Mini tube gel module 

10 x tubes 1 mm x 80mm
10 x blanking ports
Gel extraction module
2, 2mm notched glass plates
2, 2mm plain glass plates
1 Dummy plate
4 x 1mm spacers
4 x 1.5 mm spacers

2 combs 1 mm x 12 samples
2 x 1.5 mm 1D preparatory combs
Casting base

$1220.0
Maxi 2D Electrophoresis System (20 cm tank longer tubes) 
TV400YK-2DSYS 2D Electrophoresis system includes
TV400 20 cm Compact Dual Cooled Unit 
Casting base
Mini tube gel module and accessories
10 x tubes, 170 mm
10 x blanking ports
Gel extraction module
2, 4mm notched glass plates
2, 4mm plain glass plates
1 Dummy plate
4 x 1mm spacers
4 x 1.5 mm spacers
2 combs 1 mm x 24 samples
2 x 1.5 mm 1D preparatory combs

$1750.00
Power Supply
EV215 Power supply Voltage: 0...1200 V
Current: 0...500 mA
Power: 0...300 W
$1300.00
Components of this system can be purchased separately for use with existing gel tanks - see the modular electrophoresis page for more details of individual modules.

For IEF strips see the isolectric focusing system

 


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